The colorimetric estimation of plasma amino nitrogen with DNFB.
نویسنده
چکیده
A simple procedure for the estimation of plasmaamino nitrogen basedon the reaction of amino groupswith 2,4-dinitrofluorobenzene (DNFB) and subsequentmeasurement of the dinitrophenyl (DNP) amino derivatives at 420 m is presented. Elevated concentrations of bilirubin, hemoglobin, and the major nonprotein and nonamino acid nitrogen components of plasma do not seriously interfere. The proposed procedure comparesfavorably with a correspondingninhydrin technic for plasmaamino nitrogen. The mean plasma amino nitrogen values and range for a group of adults (age 18-50 years) by the proposed method is 5.56 mg./100 ml. (3.6-7.0 mg./100 ml.) compared to 4.12 mg./100 ml. (3.6-5.2 mg./100 ml.) for a group of children (age 2-10 years). Values from a group of children with sickle cell anemia showed no significant variance with the normal population. Optimum conditions for quantitation of plasma amino nitrogen along with other procedural parameters are presented.
منابع مشابه
Estimation of Alpha-amino Nitrogen in Plasma and Urine by the Colorimetric Ninhydrin Reaction.
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 14 11 شماره
صفحات -
تاریخ انتشار 1968